NSJ Bioreagents

VDAC2 Antibody

Product Code:
 
NSJ-F54504
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the VDAC2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human brain tissue with VDAC2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human kidney tissue with VDAC2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of 1) human NCI-H1299, 2) human HCT-116, 3) human PC-3, 4) mouse heart and 5) rat heart lysate with VDAC2 antibody. Predicted molecular weight: ~32 kDa.
4 / 4
Western blot testing of 1) human HCT-116, 2) human NCI-H1299, 3) human PC-3 and 4) mouse brain lysate with VDAC2 antibody. Predicted molecular weight: ~32 kDa.
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IHC testing of FFPE human brain tissue with VDAC2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with VDAC2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human NCI-H1299, 2) human HCT-116, 3) human PC-3, 4) mouse heart and 5) rat heart lysate with VDAC2 antibody. Predicted molecular weight: ~32 kDa.
Western blot testing of 1) human HCT-116, 2) human NCI-H1299, 3) human PC-3 and 4) mouse brain lysate with VDAC2 antibody. Predicted molecular weight: ~32 kDa.

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CodeSizePrice
NSJ-F54504-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54504-0.2ML0.2 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the VDAC2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation- selective.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the VDAC2 antibody.
Limitation:
This VDAC2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P45880