NSJ Bioreagents

NPY2R Antibody

Product Code:
 
NSJ-F54756
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the NPY2R antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human brain tissue with NPY2R antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) mouse brain and 2) rat brain tissue lysate with NPY2R antibody. Predicted molecular weight ~43 kDa.
3 / 4
Western blot testing of human kidney tissue lysate with NPY2R antibody. Predicted molecular weight ~43 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with NPY2R antibody; Blue=isotype control, Green= NPY2R antibody.
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IHC testing of FFPE human brain tissue with NPY2R antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) mouse brain and 2) rat brain tissue lysate with NPY2R antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human kidney tissue lysate with NPY2R antibody. Predicted molecular weight ~43 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with NPY2R antibody; Blue=isotype control, Green= NPY2R antibody.

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CodeSizePrice
NSJ-F54756-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54756-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the NPY2R antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Receptor for neuropeptide Y and peptide YY. The rank order of affinity of this receptor for pancreatic polypeptides is PYY > NPY > PYY (3-36) > NPY (2-36) > [Ile-31, Gln-34] PP > [Leu- 31, Pro-34] NPY > PP, [Pro-34] PYY and NPY free acid. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 29-65 from the human protein was used as the immunogen for the NPY2R antibody.
Limitation:
This NPY2R antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P49146