HK1 Antibody / Hexokinase 1

Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25

The stated application concentrations are suggested starting points. Titration of the Hexokinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 78-108 from the human protein was used as the immunogen for the Hexokinase antibody.

This Hexokinase antibody is available for research use only.

Antigen affinity purified

Human, Rat

P19367