NSJ Bioreagents

HIF-3 alpha Antibody / HIF3A

Product Code:
 
NSJ-RQ5626
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the HIF3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Western blot testing of 1) rat lung, 2) rat skeletal muscle, 3) mouse lung and 4) mouse NIH 3T3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
2 / 3
Western blot testing of human 1) HeLa, 2) A431, 3) Caco-2, 4) U-87 MG, 5) U-2 OS, 6) K562 and 7) PC-3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with HIF3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HIF3A antibody.
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Western blot testing of 1) rat lung, 2) rat skeletal muscle, 3) mouse lung and 4) mouse NIH 3T3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
Western blot testing of human 1) HeLa, 2) A431, 3) Caco-2, 4) U-87 MG, 5) U-2 OS, 6) K562 and 7) PC-3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
Flow cytometry testing of human U-87 MG cells with HIF3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HIF3A antibody.

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NSJ-RQ5626-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the HIF3A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q29-Q480) was used as the immunogen for the HIF3A antibody.
Limitation:
This HIF3A antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9Y2N7