NSJ Bioreagents

ADRB3 Antibody / Beta-3 Adrenergic Receptor

Product Code:
 
NSJ-RQ5645
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Beta 3 Adrenergic Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
Western blot testing of 1) human HEK293, 2) rat liver and 3) mouse liver lysate with Beta 3 Adrenergic Receptor antibody. Predicted molecular weight ~44 kDa.
2 / 4
Flow cytometry testing of human ThP-1 cells with Beta 3 Adrenergic Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta 3 Adrenergic Receptor antibody.
3 / 4
Flow cytometry testing of human HeLa cells with Beta 3 Adrenergic Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta 3 Adrenergic Receptor antibody.
4 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with Beta 3 Adrenergic Receptor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) human HEK293, 2) rat liver and 3) mouse liver lysate with Beta 3 Adrenergic Receptor antibody. Predicted molecular weight ~44 kDa.
Flow cytometry testing of human ThP-1 cells with Beta 3 Adrenergic Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta 3 Adrenergic Receptor antibody.
Flow cytometry testing of human HeLa cells with Beta 3 Adrenergic Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta 3 Adrenergic Receptor antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with Beta 3 Adrenergic Receptor antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

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CodeSizePrice
NSJ-RQ5645-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Beta 3 Adrenergic Receptor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The beta-3 adrenergic receptor, also known as ADRB3, is a beta-adrenergic receptor, and also denotes the human gene encoding it. It is mapped to 8p11.23. The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R179-S250) was used as the immunogen for the Beta 3 Adrenergic Receptor antibody.
Limitation:
This Beta 3 Adrenergic Receptor antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P13945