NSJ Bioreagents

MASP2 Antibody

Product Code:
 
NSJ-RQ6058
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MASP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of human A431 cells with MASP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MASP2 antibody.
2 / 3
Flow cytometry testing of human HepG2 cells with MASP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MASP2 antibody.
3 / 3
Western blot testing of human 1) HepG2, 2) HeLa and 3) Caco-2 lysate with MASP2 antibody. Predicted molecular weight ~76 kDa.
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Flow cytometry testing of human A431 cells with MASP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MASP2 antibody.
Flow cytometry testing of human HepG2 cells with MASP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MASP2 antibody.
Western blot testing of human 1) HepG2, 2) HeLa and 3) Caco-2 lysate with MASP2 antibody. Predicted molecular weight ~76 kDa.

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NSJ-RQ6058-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MASP2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Mannan-binding lectin serine protease 2 is a protein that in humans is encoded by the MASP2 gene. This gene is mapped to 1p36.22. This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids EAGQYGVYTKVINYIPWIENIISDF from the human protein were used as the immunogen for the MASP2 antibody.
Limitation:
This MASP2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
O00187