NSJ Bioreagents

SMPD1 Antibody / Acid Sphingomyelinase

Product Code:
 
NSJ-F54362
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Acid Sphingomyelinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) K562, 2) MCF7, 3) HepG2 and 4) A431 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
2 / 4
Western blot testing of human 1) cerebellum, 2) K562, 3) HepG2 and 4) MCF7 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
3 / 4
IHC testing of FFPE human breast tissue with Acid Sphingomyelinase antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of fixed and permeabilized human K562 cells with Acid Sphingomyelinase antibody; Blue=isotype control, Green= Acid Sphingomyelinase antibody.
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Western blot testing of human 1) K562, 2) MCF7, 3) HepG2 and 4) A431 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
Western blot testing of human 1) cerebellum, 2) K562, 3) HepG2 and 4) MCF7 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
IHC testing of FFPE human breast tissue with Acid Sphingomyelinase antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human K562 cells with Acid Sphingomyelinase antibody; Blue=isotype control, Green= Acid Sphingomyelinase antibody.

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CodeSizePrice
NSJ-F54362-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54362-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Acid Sphingomyelinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 391-419 from the human protein was used as the immunogen for the Acid Sphingomyelinase antibody.
Limitation:
This Acid Sphingomyelinase antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P17405