NSJ Bioreagents

CFH Antibody / Complement factor H

Product Code:
 
NSJ-F54343
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Complement factor H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of human 1) lung and 2) plasma lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.
2 / 4
Western blot testing of human 1) plasma and 2) kidney lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Complement factor H antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with Complement factor H antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) lung and 2) plasma lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.
Western blot testing of human 1) plasma and 2) kidney lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Complement factor H antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human hepatocellular carcinoma tissue with Complement factor H antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54343-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54343-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Complement factor H antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 751-780 from the human protein was used as the immunogen for the Complement factor H antibody.
Limitation:
This Complement factor H antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P08603