NSJ Bioreagents

DDB2 Antibody

Product Code:
 
NSJ-RQ6046
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the DDB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HeLa cells with DDB2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDB2 antibody.
2 / 2
Western blot testing of human 1) HeLa and 2) U-2 OS lysate with DDB2 antibody. Predicted molecular weight ~48 kDa.
❮❯

Flow cytometry testing of human HeLa cells with DDB2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDB2 antibody.
Western blot testing of human 1) HeLa and 2) U-2 OS lysate with DDB2 antibody. Predicted molecular weight ~48 kDa.

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NSJ-RQ6046-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DDB2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
DNA damage-binding protein 2 is a protein that in humans is encoded by the DDB2 gene. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. And this subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q10-R424) was used as the immunogen for the DDB2 antibody.
Limitation:
This DDB2 antibody is available for research use only.
PM_Meta_Key:
Product_Images
PM_Meta_Value:
https://www.nsjbio.com/get_image.php?catalog_no=RQ6046&type=image1~Flow cytometry testing of human U-87 MG cells with DDB2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DDB2 antibody.|https://www.nsjbio.com/get_image.php?catalog_no=RQ6046&type=image2~Western blot testing of human 1) HeLa, 2) A431, 3) Raji and 4) HL60 cell lysate with DDB2 antibody. Predicted molecular weight ~48 kDa.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
Q92466