NSJ Bioreagents

EDNRA Antibody / Endothelin A Receptor

Product Code:
 
NSJ-RQ5880
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Endothelin A Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
IHC staining of FFPE human lung cancer with Endothelin A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human HEK293 lysate with Endothelin A Receptor antibody. Predicted molecular weight ~49 kDa.
3 / 3
Flow cytometry testing of human A549 cells with Endothelin A Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Endothelin A Receptor antibody.
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IHC staining of FFPE human lung cancer with Endothelin A Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human HEK293 lysate with Endothelin A Receptor antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of human A549 cells with Endothelin A Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Endothelin A Receptor antibody.

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CodeSizePrice
NSJ-RQ5880-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Endothelin A Receptor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N32-Q381) was used as the immunogen for the Endothelin A Receptor antibody.
Limitation:
This Endothelin A Receptor antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P25101