NSJ Bioreagents

RHBDF2 Antibody

Product Code:
 
NSJ-F54321
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the RHBDF2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of human 1) RPMI-8226, 2) A549, 3) A431, 4) HT-1080 and 5) MCF7 cell lysate with RHBDF2 antibody. Predicted molecular weight ~97 kDa.
2 / 5
Western blot testing of human HACAT cell lysate with RHBDF2 antibody. Predicted molecular weight ~97 kDa.
3 / 5
Western blot testing of mouse lung lysate with RHBDF2 antibody. Predicted molecular weight ~97 kDa.
4 / 5
IHC testing of FFPE human duodenum tissue with RHBDF2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
IHC testing of FFPE human kidney tissue with RHBDF2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
❮❯

Western blot testing of human 1) RPMI-8226, 2) A549, 3) A431, 4) HT-1080 and 5) MCF7 cell lysate with RHBDF2 antibody. Predicted molecular weight ~97 kDa.
Western blot testing of human HACAT cell lysate with RHBDF2 antibody. Predicted molecular weight ~97 kDa.
Western blot testing of mouse lung lysate with RHBDF2 antibody. Predicted molecular weight ~97 kDa.
IHC testing of FFPE human duodenum tissue with RHBDF2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with RHBDF2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54321-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54321-0.2ML0.2 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the RHBDF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 80-109 from the human protein was used as the immunogen for the RHBDF2 antibody.
Limitation:
This RHBDF2 antibody is available for research use only.
Localization:
Cytoplasmic, plasma membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q6PJF5