NSJ Bioreagents

PRKCA Antibody / PKC alpha

Product Code:
 
NSJ-F54688
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the PRKCA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE mouse brain tissue with PRKCA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of mouse Neuro-2a cell lysate with PRKCA antibody. Predicted molecular weight ~77 kDa.
3 / 4
Western blot testing of human Jurkat cell lysate with PRKCA antibody. Predicted molecular weight ~77 kDa.
4 / 4
Flow cytometry testing of mouse Neuro-2a cells with PRKCA antibody; Left=isotype control, Right= PRKCA antibody.
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IHC testing of FFPE mouse brain tissue with PRKCA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse Neuro-2a cell lysate with PRKCA antibody. Predicted molecular weight ~77 kDa.
Western blot testing of human Jurkat cell lysate with PRKCA antibody. Predicted molecular weight ~77 kDa.
Flow cytometry testing of mouse Neuro-2a cells with PRKCA antibody; Left=isotype control, Right= PRKCA antibody.

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CodeSizePrice
NSJ-F54688-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54688-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the PRKCA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the mouse protein was used as the immunogen for the PRKCA antibody.
Limitation:
This PRKCA antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P20444