NSJ Bioreagents

Raf1 Antibody / c-Raf

Product Code:
 
NSJ-F54689
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the c-Raf antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE mouse skeletal muscle tissue with c-Raf antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of mouse Neuro-2a cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.
3 / 4
Western blot testing of human Jurkat cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.
4 / 4
Flow cytometry testing of mouse Neuro-2a cells with c-Raf antibody; Left=isotype control, Right= c-Raf antibody.
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IHC testing of FFPE mouse skeletal muscle tissue with c-Raf antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse Neuro-2a cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.
Western blot testing of human Jurkat cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.
Flow cytometry testing of mouse Neuro-2a cells with c-Raf antibody; Left=isotype control, Right= c-Raf antibody.

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CodeSizePrice
NSJ-F54689-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54689-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the c-Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 86-113 from the mouse protein was used as the immunogen for the c-Raf antibody.
Limitation:
This c-Raf antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q99N57