NSJ Bioreagents

Interferon regulatory factor 3 Antibody / IRF3

Product Code:
 
NSJ-RQ6384
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Interferon regulatory factor 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human A431 cells with Interferon regulatory factor 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) HeLa, 2) Raji, 3) HepG2 and 4) mouse lung lysate with Interferon regulatory factor 3 antibody. Predicted molecular weight ~47 kDa.
3 / 4
Flow cytometry testing of human K562 cells with Interferon regulatory factor 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Interferon regulatory factor 3 antibody.
4 / 4
IHC staining of FFPE human rectal cancer tissue with Interferon regulatory factor 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A431 cells with Interferon regulatory factor 3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) Raji, 3) HepG2 and 4) mouse lung lysate with Interferon regulatory factor 3 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human K562 cells with Interferon regulatory factor 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Interferon regulatory factor 3 antibody.
IHC staining of FFPE human rectal cancer tissue with Interferon regulatory factor 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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NSJ-RQ6384-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Interferon regulatory factor 3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. AlthoughIRF3increased transcriptional activity from an ISRE-containing promoter, expression ofIRF3as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase(CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation ofIRF3was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated withIRF3only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-S427) was used at the immunogen for the Interferon regulatory factor 3 antibody.
Limitation:
This Interferon regulatory factor 3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q14653