ATPase subunit F6 Antibody / ATP5PF
Antibody Isotype:
Rabbit IgG
Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Aliquot the ATPase subunit F6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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This product comes from:
United States.
Typical lead time:
10-14 working days.
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Further Information
Western blot: 1:500-1:2000, Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25
The stated application concentrations are suggested starting points. Titration of the ATPase subunit F6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.
Purified
In 1X PBS, pH 7.4, with 0.09% sodium azide
A portion of amino acids 28-56 from the human protein was used as the immunogen for the ATPase subunit F6 antibody.
This ATPase subunit F6 antibody is available for research use only.
Cytoplasmic
Antigen affinity purified
Human, Mouse, Rat
P18859