NSJ Bioreagents

CLK2 Antibody

Product Code:
 
NSJ-RQ5967
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CLK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human PC-3 cells with CLK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLK2 antibody.
2 / 2
Western blot testing of human 1) HeLa, 2) U-2 OS, 3) PC-3, 4) 22RV1, 5) A549, 6) COLO-320, 7) SW620, 8) rat brain, 9) rat RH35, 10) mouse HEPA1-6 and 11) mouse NIH 3T3 lysate with CLK2 antibody. Predicted molecular weight ~60 kDa.
❮❯

Flow cytometry testing of human PC-3 cells with CLK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLK2 antibody.
Western blot testing of human 1) HeLa, 2) U-2 OS, 3) PC-3, 4) 22RV1, 5) A549, 6) COLO-320, 7) SW620, 8) rat brain, 9) rat RH35, 10) mouse HEPA1-6 and 11) mouse NIH 3T3 lysate with CLK2 antibody. Predicted molecular weight ~60 kDa.

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NSJ-RQ5967-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CLK2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Dual specificity protein kinase CLK2 is an enzyme that in humans is encoded by the CLK2 gene. It is mapped to 1q22. This gene encodes a dual specificity protein kinase that phosphorylates serine/threonine and tyrosine-containing substrates. Activity of this protein regulates serine- and arginine-rich (SR) proteins of the spliceosomal complex, thereby influencing alternative transcript splicing. Chromosomal translocations have been characterized between this locus and the PAFAH1B3 (platelet-activating factor acetylhydrolase 1b, catalytic subunit 3 (29kDa)) gene on chromosome 19, resulting in the production of a fusion protein. Note that this gene is distinct from the TELO2 gene (GeneID:9894), which shares the CLK2 alias, but encodes a protein that is involved in telomere length regulation. There is a pseudogene for this gene on chromosome 7. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A140-D496) was used as the immunogen for the CLK2 antibody.
Limitation:
This CLK2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P49760