RhoF Antibody

Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Optimal dilution of the RhoF antibody should be determined by the researcher.

Lyophilized from 1X PBS with 2% Trehalose

Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described. Rho F (ras homolog gene family, member F), also known as RIF or ARHF, is a 211 amino acid membrane protein that localizes to the cytoplasmic side of the plasma membrane. Belonging to the small GTPase superfamily and the Rho family, Rho F functions cooperatively with Cdc42 and Rac to generate additional cytoskeletal structures, such as increasing variation of actin-based morphology. Rho F exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 12q24.31.

Purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Recombinant human protein (amino acids Y39-K204) was used as the immunogen for the RhoF antibody.

This RhoF antibody is available for research use only.

Antigen affinity purified

Human, Mouse, Rat

Q9HBH0