NSJ Bioreagents

cGAS Antibody / cGAMP Synthase

Product Code:
 
NSJ-RQ6412
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the cGAS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human Caco-2 cells with cGAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human A431, 4) rat lung and 5) mouse RAW264.7 cell lysate with cGAS antibody. Predicted molecular weight ~59 kDa.
3 / 4
Flow cytometry testing of human U937 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.
4 / 4
Flow cytometry testing of human ThP-1 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.
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Immunofluorescent staining of FFPE human Caco-2 cells with cGAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human A431, 4) rat lung and 5) mouse RAW264.7 cell lysate with cGAS antibody. Predicted molecular weight ~59 kDa.
Flow cytometry testing of human U937 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.
Flow cytometry testing of human ThP-1 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.

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CodeSizePrice
NSJ-RQ6412-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the cGAS antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
cGAS (cyclic GMP-AMP synthase), also known as MB21D1 (Mab-21 domain containing 1), h-cGAS or C6orf150, is a 522 amino acid cytoplasmic nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. cGAS is suggested to have antiviral activity by acting as a key cytosolic DNA sensor. cGAS binds to cytosolic DNA, which leads to cGAMP synthesis and activation of TMEM173, thereby trigger type-I interferon production. Expressed in monocytic cell line THP1, cGAS exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 6q13.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids L208-F522) was used as the immunogen for the cGAS antibody.
Limitation:
This cGAS antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8N884