NSJ Bioreagents

DNAJC6 Antibody

Product Code:
 
NSJ-RQ6170
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the DNAJC6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human A431 cells with DNAJC6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human meningioma tissue with DNAJC6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE mouse brain tissue with DNAJC6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) rat brain and 2) mouse brain lysate with DNAJC6 antibody. Predicted molecular weight ~120 kDa.
5 / 5
Flow cytometry testing of human SiHa cells with DNAJC6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNAJC6 antibody.
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Immunofluorescent staining of FFPE human A431 cells with DNAJC6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human meningioma tissue with DNAJC6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with DNAJC6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain and 2) mouse brain lysate with DNAJC6 antibody. Predicted molecular weight ~120 kDa.
Flow cytometry testing of human SiHa cells with DNAJC6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNAJC6 antibody.

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CodeSizePrice
NSJ-RQ6170-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the DNAJC6 antibody should be determined by the researcher.
Description:
Putative tyrosine-protein phosphatase auxilin is an enzyme that in humans is encoded by the DNAJC6 gene. DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids R32-Y913) was used as the immunogen for the DNAJC6 antibody.
Limitation:
This DNAJC6 antibody is available for research use only.
Localization:
Cytoplasmic
PM_Meta_Key:
Product_Images
PM_Meta_Value:
https://www.nsjbio.com/get_image.php?catalog_no=RQ6170&type=image1~Immunofluorescent staining of FFPE human A431 cells with DNAJC6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.|https://www.nsjbio.com/get_image.php?catalog_no=RQ6170&type=image2~Immunofluorescent staining of FFPE human A549 cells with DNAJC6 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.|https://www.nsjbio.com/get_image.php?catalog_no=RQ6170&type=image3~IHC staining of FFPE rat brain tissue with DNAJC6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|https://www.nsjbio.com/get_image.php?catalog_no=RQ6170&type=image4~Western blot testing of 1) rat brain and 2) mouse brain lysate with DNAJC6 antibody. Predicted molecular weight ~120 kDa.|https://www.nsjbio.com/get_image.php?catalog_no=RQ6170&type=image5~Western blot testing of 1) human HEL, 2) human K562, 3) human SH-SY5Y, 4) rat brain, 5) rat PC-12 and 6) mouse brain tissue lysate with DNAJC6 antibody. Predicted molecular weight ~120 kDa.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75061