NSJ Bioreagents

Cadherin 11 Antibody / CDH11

Product Code:
 
NSJ-RQ6247
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Cadherin 11 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human prostate cancer with Cadherin 11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human liver cancer with Cadherin 11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human PC-3, 2) rat brain and 3) mouse brain lysate with Cadherin 11 antibody. Predicted molecular weight ~120 kDa.
4 / 4
Flow cytometry testing of human PC-3 cells with Cadherin 11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OB-Cadherin antibody.
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IHC staining of FFPE human prostate cancer with Cadherin 11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Cadherin 11 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human PC-3, 2) rat brain and 3) mouse brain lysate with Cadherin 11 antibody. Predicted molecular weight ~120 kDa.
Flow cytometry testing of human PC-3 cells with Cadherin 11 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OB-Cadherin antibody.

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CodeSizePrice
NSJ-RQ6247-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cadherin 11 antibody should be determined by the researcher.
Description:
Cadherin-11, also called Osteoblast cadherin or OB Cadherin, is a protein that in humans is encoded by the CDH11 gene. This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids K371-S796) was used as the immunogen for the Cadherin 11 antibody.
Limitation:
This Cadherin 11 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P55287