NSJ Bioreagents

GRB10 Antibody

Product Code:
 
NSJ-RQ5730
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the GRB10 antibody can be stored for up to one month at 4oC. For long-term aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 7
Western blot testing of 1) human HEK293, 2) HeLa, 3) rat kidney and 4) mouse kidney lysate with GRB10 antibody. Expected molecular weight: 58-70 kDa.
2 / 7
Western blot testing of human 1) human HeLa, 2) human HepG2, 3) human 293T, 4) monkey COS-7, 5) human K562, 6) human U-87 MG, 7) human HEL, 8) rat liver, 9) rat brain, 10) mouse liver and 11) mouse brain tissue lysate with GRB10 antibody. Expected molecular weight: 58-70 kDa.
3 / 7
IHC staining of FFPE human liver cancer tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE human colorectal adenocarcinoma tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE mouse brain tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 7
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with GRB10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GRB10 antibody.
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Western blot testing of 1) human HEK293, 2) HeLa, 3) rat kidney and 4) mouse kidney lysate with GRB10 antibody. Expected molecular weight: 58-70 kDa.
Western blot testing of human 1) human HeLa, 2) human HepG2, 3) human 293T, 4) monkey COS-7, 5) human K562, 6) human U-87 MG, 7) human HEL, 8) rat liver, 9) rat brain, 10) mouse liver and 11) mouse brain tissue lysate with GRB10 antibody. Expected molecular weight: 58-70 kDa.
IHC staining of FFPE human liver cancer tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with GRB10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with GRB10 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GRB10 antibody.

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CodeSizePrice
NSJ-RQ5730-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry: 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the GRB10 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
GRB10, Growth factor receptor-bound protein 10, also known as insulin receptor-binding protein Grb-IR is a protein that in humans is encoded by the GRB10 gene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors(e.g., IGF1R and IGF2R). Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-K251) was used as the immunogen for the GRB10 antibody.
Limitation:
This GRB10 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13322