NSJ Bioreagents

PHEX Antibody / PEX / HYP

Product Code:
 
NSJ-RQ5999
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PHEX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human ovarian cancer with PHEX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human lung cancer with PHEX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Flow cytometry testing of human A549 cells with PHEX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHEX antibody.
4 / 4
Western blot testing of 1) rat ovary, 2) rat kidney, 3) mouse ovary, 4) mouse kidney, 5) human HEK293, 6) monkey kidney, 7) rat NRK and 8) monkey COS-7 cell lysate with PHEX antibody. Predicted molecular weight ~86 kDa.
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IHC staining of FFPE human ovarian cancer with PHEX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with PHEX antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human A549 cells with PHEX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHEX antibody.
Western blot testing of 1) rat ovary, 2) rat kidney, 3) mouse ovary, 4) mouse kidney, 5) human HEK293, 6) monkey kidney, 7) rat NRK and 8) monkey COS-7 cell lysate with PHEX antibody. Predicted molecular weight ~86 kDa.

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CodeSizePrice
NSJ-RQ5999-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PHEX antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Phosphate-regulating neutral endopeptidase, X-linked also known as phosphate-regulating gene with homologies to endopeptidases on the X chromosome or metalloendopeptidase homolog PEX is an enzyme that in humans is encoded by the PHEX gene. It is mapped to Xp22.11. The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E145-N718) was used as the immunogen for the PHEX antibody.
Limitation:
This PHEX antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P78562