NSJ Bioreagents

DHX9 Antibody / RNA Helicase A

Product Code:
 
NSJ-RQ5970
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RNA Helicase A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 10
Immunofluorescent staining of FFPE human gastric cancer with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
2 / 10
Immunofluorescent staining of FFPE human U-2 OS cells with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 10
IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human renal cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
Western blot testing of human 1) HeLa, 2) HEK293, 3) Jurkat, 4) K562, 5) Caco-2, 6) SW620, 7) Raji and 8) A549 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.
9 / 10
Western blot testing of 1) rat PC-12, 2) mouse brain and 3) mouse RAW264.7 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.
10 / 10
Flow cytometry testing of human HL60 cells with RNA Helicase A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNA Helicase A antibody.
❮❯

Immunofluorescent staining of FFPE human gastric cancer with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HEK293, 3) Jurkat, 4) K562, 5) Caco-2, 6) SW620, 7) Raji and 8) A549 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.
Western blot testing of 1) rat PC-12, 2) mouse brain and 3) mouse RAW264.7 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.
Flow cytometry testing of human HL60 cells with RNA Helicase A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNA Helicase A antibody.

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NSJ-RQ5970-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RNA Helicase A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
ATP-dependent RNA helicase A (RHA; also known as DHX9, LKP, and NDHI) is an enzyme that in humans is encoded by the DHX9 gene. It is mapped to 1q25.3. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids E396-E759) was used as the immunogen for the RNA Helicase A antibody.
Limitation:
This RNA Helicase A antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q08211