NSJ Bioreagents

PI16 Antibody

Product Code:
 
NSJ-RQ6148
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PI16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 8
IHC staining of FFPE human testicular cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE human pancreatic cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human bladder cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE mouse intestine with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE rat intestine with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Western blot testing of 1) HeLa, 2) human Jurkat, 3) human PC-3, 4) monkey heart, 5) rat heart, 6) rat testis and 7) mouse heart lysate with PI16 antibody. Predicted molecular weight ~50 kDa, routinely observed at 70-110 kDa depending on glycosylation level.
7 / 8
Flow cytometry testing of human A431 cells with PI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI16 antibody.
8 / 8
Flow cytometry testing of mouse HEPA1-6 cells with PI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI16 antibody.
❮❯

IHC staining of FFPE human testicular cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder cancer with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with PI16 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) HeLa, 2) human Jurkat, 3) human PC-3, 4) monkey heart, 5) rat heart, 6) rat testis and 7) mouse heart lysate with PI16 antibody. Predicted molecular weight ~50 kDa, routinely observed at 70-110 kDa depending on glycosylation level.
Flow cytometry testing of human A431 cells with PI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI16 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with PI16 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI16 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ6148-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PI16 antibody should be determined by the researcher.
Description:
Peptidase inhibitor 16 is a protein that in humans is encoded by the PI16 gene. PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids L28-L363) was used as the immunogen for the PI16 antibody.
Limitation:
This PI16 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
Q6UXB8