NSJ Bioreagents

ADRA1D Antibody / Alpha Adrenergic Receptor 1d

Product Code:
 
NSJ-F54388
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the ADRA1D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human small intestine tissue with ADRA1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human kidney tissue with ADRA1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC testing of FFPE rat kidney tissue with ADRA1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Western blot testing of human 1) A549, 2) LNCaP, 3) PC-3 and 4) rat brain lysate with ADRA1D antibody. Predicted molecular weight ~60 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human MCF7 cells with ADRA1D antibody; Blue=isotype control, Green= ADRA1D antibody.
❮❯

IHC testing of FFPE human small intestine tissue with ADRA1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with ADRA1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat kidney tissue with ADRA1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) A549, 2) LNCaP, 3) PC-3 and 4) rat brain lysate with ADRA1D antibody. Predicted molecular weight ~60 kDa.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with ADRA1D antibody; Blue=isotype control, Green= ADRA1D antibody.

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CodeSizePrice
NSJ-F54388-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54388-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the ADRA1D antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the ADRA1D antibody.
Limitation:
This ADRA1D antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P25100