AdipoGen Life Sciences

anti-beta-Tubulin mAb (AXO45)

Product Code:
 
AG-20B-0085
Product Group:
 
Primary Antibodies
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AXO45
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
BLUE ICE
Storage:
 
Short Term Storage: +4?C. Long Term Storage: -20?C
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Figure 1: Immunoblot analysis of ?-tubulin protein glutamylation using anti-?-Tubulin, mAb (AXO45) (Prod. No. AG-20B-0085). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases, TTLL4 and TTLL6 and are run on a 10% SDS-PAGE (specific protocol to separate ?- and ?-tubulin (Magiera & Janke, 2013)). The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the AXO45 (1:1'000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In all the cell extracts, AXO45 specifically detects ?-tubulin, with no cross-reactivity to ?-tubulin. Glutamylation added by either TTLL4 or TTLL6 appears to not affect this detection. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.
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Figure 1: Immunoblot analysis of ?-tubulin protein glutamylation using anti-?-Tubulin, mAb (AXO45) (Prod. No. AG-20B-0085). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases, TTLL4 and TTLL6 and are run on a 10% SDS-PAGE (specific protocol to separate ?- and ?-tubulin (Magiera & Janke, 2013)). The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the AXO45 (1:1'000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In all the cell extracts, AXO45 specifically detects ?-tubulin, with no cross-reactivity to ?-tubulin. Glutamylation added by either TTLL4 or TTLL6 appears to not affect this detection. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.

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Further Information

Alternate Names/Synonyms:
Tubulin beta Chain; Tubulin beta-5 Chain
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 0.02% Proclin 300.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Immunogen:
Axonemal tubulin from the ciliate Paramecium electroeluted from the 55 kDa band of the SDS-PAGE gel.
Long Description:
Monoclonal Antibody. Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates. Applications: ICC, IHC, IP and WB. Clone: AXO45. Isotype: Mouse IgG1. The β-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin (A.M. Callen, et al.; 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals.
Package Type:
Plastic Vial
Product Description:
The beta-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both alpha- and beta-tubulin (A.M. Callen, et al.; 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of beta-tubulin. Although the antibody was raised against the beta-tubulin found in cilia, it is able to recognize beta-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of beta-tubulin found in mammals.
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Detects almost all isotypes of beta-tubulin specifically in cells and tissues of mouse, human and ciliates.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

References

Isolation and characterization of libraries of monoclonal antibodies directed against various forms of tubulin in Paramecium: A.M. Callen, et al.; Biol. Cell 81, 95 (1994) | A targeted multienzyme mechanism for selective microtubule polyglutamylation: J. van Dijk, et al.; Mol. Cell 26, 437 (2007) | Investigating tubulin posttranslational modifications with specific antibodies: M.M. Magiera, et al.; Methods Cell Biol. 115, 247 (2013)