ProSci

MNDA (CT) Antibody

Product Code:
 
PSI-9645
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
MNDA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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<strong>Figure 1 Western Blot Validation in Human THP-1 Cells </strong><br> Loading: 15 μg of lysate per lane. Antibodies: MNDA 9645, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 μg/mL Lane 2: 2 μg/mL
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<strong>Figure 2 Immunohistochemistry Validation of MNDA in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MNDA antibody (9645) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Western Blot Validation in Human THP-1 Cells </strong><br> Loading: 15 μg of lysate per lane. Antibodies: MNDA 9645, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 1 μg/mL Lane 2: 2 μg/mL
<strong>Figure 2 Immunohistochemistry Validation of MNDA in Human Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MNDA antibody (9645) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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PSI-9645-0.02mg0.02mg£150.00
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PSI-9645-0.1mg0.1mg£449.00
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Further Information

Additional Names:
MNDA
Application Note:
WB: 1 μg/mL; IHC: 2 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
MNDA Antibody: MNDA controls the expression of MCL-1 and BCL02 in chronic lymphocytic leukemia cells. MNDA is also a useful marker in the recognition and differential diagnosis of marginal zone lymphomas. MNDA has a role in regulating programmed cell death in myeloid progenitor cells, and its down-regulation in myelodysplastic syndromes is related to granulocyte-macrophage progenitor cell sensitivity to TRAIL-induced programmed cell death.
Background References:
  • Bottari et al. Exp Hematol. 2020; 88:68-82
  • Wang et al. Am. J. Clin. Pathol. 2019; 151(3):337-343
  • Kanellis et al. Lukemia 2019; 23(10):1847-1857
  • Briggs et al. Cancer Res. 2006; 66(9): 4645-4651
Buffer:
MNDA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
Homology:
Predicted species reactivity based on immunogen sequence: Gorilla (100%), chimpanzee (100%), Monkey (92%)
Immunogen:
Anti-MNDA antibody (9645) was raised against a peptide corresponding to 13 amino acids near the carboxyl terminus of human MNDA. The immunogen is located within the last 50 amino acids of MNDA.
ISOFORMS:
Human MNDA has one isoform (407aa, 45.8kD). Mouse MNDA has 2 isoforms, isoform 1 (425aa, 47kD) and isoform 2 (423aa, 46.8kD). Rat MNDA has one isoform (438aa, 49.7kD). 9645 can detect MNDA in human, but not in mouse and rat.
NCBI Gene ID #:
4332
NCBI Official Name:
myeloid cell nuclear differentiation antigen
NCBI Official Symbol:
MNDA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46kD

Observed: 46 kD
Protein Accession #:
P41218.1
Protein GI Number:
730038
Purification:
MNDA Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
P41218.1
User NOte:
Optimal dilutions for each application to be determined by the researcher.