Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
PEX3 antibody can be stored at 4 ˚C for three months and -20 ˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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United States.
Typical lead time:
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Further Information
PEX3 Antibody: Peroxisomal biogenesis factor 3, Peroxin-3, Peroxisomal assembly protein PEX3
WB: 2 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
PEX3 Antibody: PEX3 is involved in peroxisome biosynthesis and integrity. It assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, PEX3 is necessary for the import of peroxisomal membrane proteins in the peroxisomes. Thus within the cell, PEX3 is stabilized by PEX19 preventing PEX3 aggregation. Mutations in the PEX19-binding domain of PEX3 reduce the affinity for PEX19 and destabilize PEX3.
- Ghaedi et al. Mol Biol Cell. 2000; 11(6):2085-2102
- Fang et al. J Cell Biol. 2004;164(6):863-875
- Hattula et al. PloS One. 2014; 9(7):e103101
- Schmidt et al. Traffic. 2012; 13(9):1244-1260
PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Predicted species reactivity based on immunogen sequence: monkey (100%), cow (100%)
Anti-PEX3 antibody (9665) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminal of human PEX3. The immunogen is located within last 50 amino acids of PEX3.
Human PEX3 has 1 isoform, including isoform 1 (373aa, 42kD). Mouse PEX3 has 1 isoform (372aa, 42kD). Rat PEX3 has one isoform (372aa, 42kD). 9665 can detect PEX3 in human, mouse and rat.
8504
PEX3
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 42kD
Observed: 42 kD
P56589
3914303
PEX3 Antibody is affinity chromatography purified via peptide column.
Signal Transduction
P56589
Optimal dilutions for each application to be determined by the researcher.