NSJ Bioreagents

Prohibitin 2 Antibody / PHB2

Product Code:
 
NSJ-F54917
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Prohibitin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human colon tissue with Prohibitin 2 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human kidney tissue with Prohibitin 2 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human brain tissue with Prohibitin 2 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
4 / 4
Western blot testing of 1) human Raji, 2) human HEK293, 3) human HeLa, 4) human MDA-MB-453, 5) mouse NIH 3T3, 6) mouse brain and 7) rat C6 cell lysate with Prohibitin 2 antibody. Predicted molecular weight ~33 kDa.
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IHC testing of FFPE human colon tissue with Prohibitin 2 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with Prohibitin 2 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with Prohibitin 2 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human Raji, 2) human HEK293, 3) human HeLa, 4) human MDA-MB-453, 5) mouse NIH 3T3, 6) mouse brain and 7) rat C6 cell lysate with Prohibitin 2 antibody. Predicted molecular weight ~33 kDa.

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CodeSizePrice
NSJ-F54917-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54917-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 225-255 from the human protein was used as the immunogen for the Prohibitin 2 antibody.
Limitation:
This Prohibitin 2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99623