NSJ Bioreagents

PSMB9 Antibody / Proteasome subunit beta type-9

Product Code:
 
NSJ-F54978
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the PSMB9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human prostate carcinoma tissue with PSMB9 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human HL60 cell lysate with PSMB9 antibody. Predicted molecular weight ~23 kDa.
3 / 4
Western blot testing of human 1) A431 and 2) Raji cell lysate with PSMB9 antibody. Predicted molecular weight ~23 kDa.
4 / 4
Flow cytometry testing of human HL60 cells with PSMB9 antibody; Blue=isotype control, Green= PSMB9 antibody.
❮❯

IHC testing of FFPE human prostate carcinoma tissue with PSMB9 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HL60 cell lysate with PSMB9 antibody. Predicted molecular weight ~23 kDa.
Western blot testing of human 1) A431 and 2) Raji cell lysate with PSMB9 antibody. Predicted molecular weight ~23 kDa.
Flow cytometry testing of human HL60 cells with PSMB9 antibody; Blue=isotype control, Green= PSMB9 antibody.

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CodeSizePrice
NSJ-F54978-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54978-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 193-219 from the human protein was used as the immunogen for the PSMB9 antibody.
Limitation:
This PSMB9 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P28065