NSJ Bioreagents

FAK Antibody / Focal Adhesion Kinase / PTK2

Product Code:
 
NSJ-F54983
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the PTK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human brain tissue with PTK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HEK293 cells with PTK2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PTK2 antibody.
4 / 4
Flow cytometry testing of human K562 cells with PTK2 antibody; Blue=isotype control, Green= PTK2 antibody.
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IHC testing of FFPE human brain tissue with PTK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HEK293 cells with PTK2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PTK2 antibody.
Flow cytometry testing of human K562 cells with PTK2 antibody; Blue=isotype control, Green= PTK2 antibody.

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CodeSizePrice
NSJ-F54983-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54983-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 821-848 from the human protein was used as the immunogen for the PTK2 antibody.
Limitation:
This PTK2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear, extracellular
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q05397