NSJ Bioreagents

Cdc37 Antibody

Product Code:
 
NSJ-F55012
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Cdc37 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of 1) human A431, 2) rat C6, 3) human Jurkat, 4) human K562 and 5) human LNCaP cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
2 / 4
Western blot testing of human 1) MCF7 and 2) A2058 cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
3 / 4
IHC testing of FFPE human skin tissue with Cdc37 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human MCF7 cells with Cdc37 antibody; Blue=isotype control, Green= Cdc37 antibody.
❮❯

Western blot testing of 1) human A431, 2) rat C6, 3) human Jurkat, 4) human K562 and 5) human LNCaP cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
Western blot testing of human 1) MCF7 and 2) A2058 cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
IHC testing of FFPE human skin tissue with Cdc37 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MCF7 cells with Cdc37 antibody; Blue=isotype control, Green= Cdc37 antibody.

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CodeSizePrice
NSJ-F55012-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55012-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:1000-1:2000
Description:
Cdc37 is a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 116-144 from the human protein was used as the immunogen for the Cdc37 antibody.
Limitation:
This Cdc37 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
Q16543