NSJ Bioreagents

WT1 Antibody / Wilms Tumor 1

Product Code:
 
NSJ-V9173
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rWT1/6908
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant WT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human ovarian carcinoma tissue with recombinant WT1 antibody (clone rWT1/6908). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
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IHC staining of FFPE human ovarian carcinoma tissue with recombinant WT1 antibody (clone rWT1/6908). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~

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CodeSizePrice
NSJ-V9173-20UG20ug£264.00
Quantity:
NSJ-V9173-100UG100ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.
Description:
The WT1 gene located at chromosome 11p13 codes for a transcription factor, a DNA-binding nucleoprotein, that plays a role primarily in the development of genitourinary organs. There are at least eight isoforms ranging between 52 and 62kDa produced by a combination of alternative splicing and RNA editing. WT1 is synthesized and reside in the cytoplasm in an inactive form. When activated through phosphorylation it is translocated to the nucleus. In tumor tissues, WT1 is detected in tumor cells of Wilms Tumor (also known as nephroblastoma) and mesothelioma. Additionally, WT1 expression has been found in ovarian serous carcinomas and some breast carcinomas. WT1 is particularly used for distinguishing malignant mesothelioma and ovarian serous carcinoma from non-serous carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 1-100 was used as the immunogen for the recombinant WT1 antibody.
Limitation:
This recombinant WT1 antibody is available for research use only.
Localization:
Nucleus and cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P19544