NSJ Bioreagents

Bcl-2 Antibody

Product Code:
 
NSJ-V9288
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
BCL2/6426R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Bcl-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human tonsil tissue with recombinant Bcl-2 antibody (clone BCL2/6426R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with recombinant Bcl-2 antibody (clone BCL2/6426R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V9288-20UG20ug£264.00
Quantity:
NSJ-V9288-100UG100ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Bcl-2 antibody should be determined by the researcher.
Description:
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for the recombinant Bcl-2 antibody.
Limitation:
This recombinant Bcl-2 antibody is available for research use only.
Localization:
Cytoplasm, nuclear membrane
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P10415