NSJ Bioreagents

CEA Antibody / Carcinoembryonic Antigen

Product Code:
 
NSJ-V9318
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
C66/6470R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Carcinoembryonic Antigen antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human colon tissue with recombinant Carcinoembryonic Antigen antibody (clone C66/6470R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Negative control: IHC staining of FFPE human brain tissue using recombinant Carcinoembryonic Antigen antibody (clone C66/6470R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Carcinoembryonic Antigen antibody (clone C66/6470R) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human colon tissue with recombinant Carcinoembryonic Antigen antibody (clone C66/6470R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant Carcinoembryonic Antigen antibody (clone C66/6470R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Carcinoembryonic Antigen antibody (clone C66/6470R) as confirmation of integrity and purity.

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NSJ-V9318-20UG20ug£264.00
Quantity:
NSJ-V9318-100UG100ug£534.00
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Carcinoembryonic Antigen antibody should be determined by the researcher.
Description:
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human CEA protein was used as the immunogen for the recombinant Carcinoembryonic Antigen antibody.
Limitation:
This recombinant Carcinoembryonic Antigen antibody is available for research use only.
Localization:
Cytoplasm and luminal surface
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P06731