NSJ Bioreagents

IDH1 Antibody / Isocitrate Dehydrogenase (R132H mutant)

Product Code:
 
NSJ-V9378
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
IDH1/6806R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the IDH1 R132H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of IDH1-R132H mutation protein in FFPE human prostate carcinoma tissue with IDH1-R132H antibody (clone IDH1/6806R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human glioblastoma tissue with IDH1-R132H antibody (clone IDH1/6806R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Negative control: IHC staining of FFPE human cerebellum tissue with IDH1 R132H antibody (clone IDH1/6806R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of IDH1-R132H mutation protein in FFPE human prostate carcinoma tissue with IDH1-R132H antibody (clone IDH1/6806R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human glioblastoma tissue with IDH1-R132H antibody (clone IDH1/6806R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human cerebellum tissue with IDH1 R132H antibody (clone IDH1/6806R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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NSJ-V9378-20UG20ug£264.00
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NSJ-V9378-100UG100ug£534.00
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the IDH1 R132H antibody should be determined by the researcher.
Description:
IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 281-414 was used as the immunogen for the IDH1 R132H antibody.
Limitation:
This IDH1 R132H antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
O75874