NSJ Bioreagents

Filaggrin Antibody

Product Code:
 
NSJ-V9389
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rFLG/1945
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant FLG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human skin tissue with recombinant FLG antibody (clone rFLG/1945). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human skin tissue with recombinant FLG antibody (clone rFLG/1945). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V9389-20UG20ug£264.00
Quantity:
NSJ-V9389-100UG100ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant FLG antibody should be determined by the researcher.
Description:
Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 198-288 was used as the immunogen for the recombinant FLG antibody.
Limitation:
This recombinant FLG antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P20930