NSJ Bioreagents

Leptin Receptor Antibody / LEPR

Product Code:
 
NSJ-V9168
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LEPR/4301
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Leptin Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Leptin Receptor antibody (clone LEPR/4301). These results demonstrate the foremost specificity of the LEPR/4301 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 3
IHC staining of human liver tissue with Leptin Receptor antibody (clone LEPR/4301). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free Leptin Receptor antibody (clone LEPR/4301) as confirmation of integrity and purity.
❮❯

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Leptin Receptor antibody (clone LEPR/4301). These results demonstrate the foremost specificity of the LEPR/4301 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of human liver tissue with Leptin Receptor antibody (clone LEPR/4301). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Leptin Receptor antibody (clone LEPR/4301) as confirmation of integrity and purity.

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NSJ-V9168-20UG20ug£264.00
Quantity:
NSJ-V9168-100UG100ug£534.00
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Leptin Receptor antibody should be determined by the researcher.
Description:
The Ob gene encodes the protein leptin. The leptin receptor, designated Ob-R, has been shown to be a single membrane-spanning receptor that most resembles the gp130 signal transducing component of the IL-6, G-CSF and LIF receptor.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 335-425 was used as the immunogen for the Leptin Receptor antibody.
Limitation:
This Leptin Receptor antibody is available for research use only.
Localization:
Secreted, Cell surface
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P48357