NSJ Bioreagents

TARBP2 Antibody

Product Code:
 
NSJ-V9245
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-TARBP2-1E5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the TARBP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using TARBP2 antibody (green, clone PCRP-TARBP2-1E5) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with TARBP2 antibody (blue, clone PCRP-TARBP2-1E5), and unstained cells (red).
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TARBP2 antibody (clone PCRP-TARBP2-1E5). These results demonstrate the foremost specificity of the PCRP-TARBP2-1E5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free TARBP2 antibody (clone PCRP-TARBP2-1E5) as confirmation of integrity and purity.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using TARBP2 antibody (green, clone PCRP-TARBP2-1E5) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with TARBP2 antibody (blue, clone PCRP-TARBP2-1E5), and unstained cells (red).
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using TARBP2 antibody (clone PCRP-TARBP2-1E5). These results demonstrate the foremost specificity of the PCRP-TARBP2-1E5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free TARBP2 antibody (clone PCRP-TARBP2-1E5) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9245-20UG20ug£264.00
Quantity:
NSJ-V9245-100UG100ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the TARBP2 antibody should be determined by the researcher.
Description:
TRBP2, also known as TARBP2 (trans-activation-responsive (HIV-1) RNA binding protein 2), TRBP1 or TRBP, is a nuclear protein that contains three DRBM (double-stranded RNA-binding) domains. TRBP binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. The third DRBM motif in the C-terminus of human TRBP2 can interact with and inhibit PKR activity, thereby increasing HIV-1 long terminal repeat (LTR) expression. In addition, TRBP2 functions as a component of a Dicer-containing complex and associates with the catalytic subunit of the RNA-induced silencing complex (RISC), namely eIF2C2. TRBP2 is essential for Dicer stability and the proper assembly of RISC. This suggests that TRBP2, in association with Dicer, plays an important role in the processing of miRNAs (microRNAs).
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human TARBP2 protein was used as the immunogen for the TARBP2 antibody.
Limitation:
This TARBP2 antibody is available for research use only.
Localization:
Cytoplasm, Nucleus
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q15633