NSJ Bioreagents

Glutamine Synthetase Antibody / GLUL

Product Code:
 
NSJ-V8915
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2bc, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
GLUL/6600
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Glutamine Synthetase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of human liver tissue lysate using Glutamine Synthetase antibody (clone GLUL/6600). Predicted molecular weight ~42 kDa.
2 / 3
Western blot testing of human Y79 cell lysate using Glutamine Synthetase antibody (clone GLUL/6600). Predicted molecular weight ~42 kDa.
3 / 3
IHC staining of FFPE human stomach tissue with Glutamine Synthetase antibody (clone GLUL/6600) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human liver tissue lysate using Glutamine Synthetase antibody (clone GLUL/6600). Predicted molecular weight ~42 kDa.
Western blot testing of human Y79 cell lysate using Glutamine Synthetase antibody (clone GLUL/6600). Predicted molecular weight ~42 kDa.
IHC staining of FFPE human stomach tissue with Glutamine Synthetase antibody (clone GLUL/6600) at 2ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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NSJ-V8915-20UG20ug£264.00
Quantity:
NSJ-V8915-100UG100ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Glutamine Synthetase antibody should be determined by the researcher.
Description:
Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 50-250 was used as the immunogen for the Glutamine Synthetase antibody.
Limitation:
This Glutamine Synthetase antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P15104