NSJ Bioreagents

Daxx Antibody

Product Code:
 
NSJ-V8935
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-DAXX-8C2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Daxx antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using Daxx antibody (green, clone PCRP-DAXX-8C2).
2 / 4
FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8C2), and unstained cells (red).
3 / 4
IHC staining of FFPE human kidney tissue with Daxx antibody (clone PCRP-DAXX-8C2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8C2) as confirmation of integrity and purity.
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Immunofluorescent staining of PFA-fixed human HeLa cells using Daxx antibody (green, clone PCRP-DAXX-8C2).
FACS staining of PFA-fixed human HeLa cells with Daxx antibody (blue, clone PCRP-DAXX-8C2), and unstained cells (red).
IHC staining of FFPE human kidney tissue with Daxx antibody (clone PCRP-DAXX-8C2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Daxx antibody (clone PCRP-DAXX-8C2) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8935-20UG20ug£264.00
Quantity:
NSJ-V8935-100UG100ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Daxx antibody should be determined by the researcher.
Description:
Daxx, or Death domain-associated protein 6, is a transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human Death domain-associated protein 6 protein was used as the immunogen for the Daxx antibody.
Limitation:
This Daxx antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q9UER7