NSJ Bioreagents

BMP4 Antibody / Bone morphogenetic protein 4

Product Code:
 
NSJ-RQ5387
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BBB-2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the BMP4 antibody at -20oC.
1 / 2
IHC staining of FFPE human breast cancer with BMP4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of human HeLa cell lysate with BMP4 antibody. Expected molecular weight: 54 kDa (precursor), 44 kDa (cleaved dimer), 23 kDa (cleaved monomer).
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IHC staining of FFPE human breast cancer with BMP4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human HeLa cell lysate with BMP4 antibody. Expected molecular weight: 54 kDa (precursor), 44 kDa (cleaved dimer), 23 kDa (cleaved monomer).

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NSJ-RQ5387-100ul100 ul£535.00
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the BMP4 antibody should be determined by the researcher.
Description:
The BMP4 gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human BMP4 was used as the immunogen for the BMP4 antibody.
Limitation:
This BMP4 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P12644