NSJ Bioreagents

NQO1 Antibody

Product Code:
 
NSJ-RQ5489
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AAOB-14
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store the NQO1 antibody at -20oC.
1 / 5
Western blot testing of human SH-SY5Y cell lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.
2 / 5
Immunofluorescent staining of FFPE human MCF7 cells with NQO1 antibody (green) and DAPI nuclear stain (blue).
3 / 5
Western blot testing of human 1) HeLa, 2) HepG2 and 3) MCF7 cell lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.
4 / 5
Western blot testing of mouse kidney tissue lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.
5 / 5
Western blot testing of rat 1) liver, 2) spleen and 3) lung tissue lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.
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Western blot testing of human SH-SY5Y cell lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.
Immunofluorescent staining of FFPE human MCF7 cells with NQO1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) HeLa, 2) HepG2 and 3) MCF7 cell lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.
Western blot testing of mouse kidney tissue lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.
Western blot testing of rat 1) liver, 2) spleen and 3) lung tissue lysate with NQO1 antibody. Predicted molecular weight ~30 kDa.

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NSJ-RQ5489-100ul100 ul£535.00
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Further Information

Application Details :
Western blot: 1:1000-1:5000,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the NQO1 antibody should be determined by the researcher.
Description:
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human NQO1 was used as the immunogen for the NQO1 antibody.
Limitation:
This NQO1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P15559