NSJ Bioreagents

UBA2 Antibody / SUMO-activating enzyme subunit 2 / SAE2

Product Code:
 
NSJ-RQ5505
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
5H11
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SAE2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of human A431 cells with SAE2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SAE2 antibody.
2 / 3
Flow cytometry testing of human U-2 OS cells with SAE2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SAE2 antibody.
3 / 3
Western blot testing of human 1) K562, 2) Raji, 3) ThP-1, 4) SW579, 5) HepG2, 6) CCRF-CEM, 7) rat PC-12 and 8) mouse RAW246.7 lysate with SAE2 antibody. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.
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Flow cytometry testing of human A431 cells with SAE2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SAE2 antibody.
Flow cytometry testing of human U-2 OS cells with SAE2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SAE2 antibody.
Western blot testing of human 1) K562, 2) Raji, 3) ThP-1, 4) SW579, 5) HepG2, 6) CCRF-CEM, 7) rat PC-12 and 8) mouse RAW246.7 lysate with SAE2 antibody. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.

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CodeSizePrice
NSJ-RQ5505-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the SAE2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids E449-K564) was used as the immunogen for the SAE2 antibody.
Limitation:
This SAE2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UBT2