NSJ Bioreagents

COMT Antibody

Product Code:
 
NSJ-RQ5527
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
15C10
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the COMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 11
Immunofluorescent staining of FFPE human A431 cells with COMT antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 11
Flow cytometry testing of human K562 cells with COMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COMT antibody.
3 / 11
Flow cytometry testing of human U-87 MG cells with COMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COMT antibody.
4 / 11
Western blot testing of human 1) HepG2, 2) K562, 3) ThP-1, 4) HEK293, 5) A549, 6) Caco-2, 7) rat RH35 and 8) mouse Neuro-2a lysate with COMT antibody. Predicted molecular weight: ~30/25 kDa (isoforms 1/2).
5 / 11
IHC staining of FFPE human breast cancer with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human placenta with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human intestinal cancer with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human lung cancer with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 11
IHC staining of FFPE mouse testis with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
10 / 11
IHC staining of FFPE rat testis with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
11 / 11
Immunofluorescent staining of FFPE human MCF7 cells with COMT antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A431 cells with COMT antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human K562 cells with COMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COMT antibody.
Flow cytometry testing of human U-87 MG cells with COMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= COMT antibody.
Western blot testing of human 1) HepG2, 2) K562, 3) ThP-1, 4) HEK293, 5) A549, 6) Caco-2, 7) rat RH35 and 8) mouse Neuro-2a lysate with COMT antibody. Predicted molecular weight: ~30/25 kDa (isoforms 1/2).
IHC staining of FFPE human breast cancer with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis with COMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with COMT antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-RQ5527-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml, Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the COMT antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids G52-P271) was used as the immunogen for the COMT antibody.
Limitation:
This COMT antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P21964