NSJ Bioreagents

SIRT1 Antibody

Product Code:
 
NSJ-RQ5549
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SIRT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 6
IHC staining of FFPE human intestinal cancer with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE mouse testis with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE rat testis with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human U-2 OS cells with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) rat testis, 2) mouse thymus, 3) mouse testis, 4) mouse NIH3T3 and 5) human HL-60 lysate with SIRT1 antibody. Predicted molecular weight ~80 kDa but is routinely observed at 110~120 kDa due to post-translational modification.
6 / 6
Flow cytometry testing of human HeLa cells with SIRT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIRT1 antibody.
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IHC staining of FFPE human intestinal cancer with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with SIRT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) rat testis, 2) mouse thymus, 3) mouse testis, 4) mouse NIH3T3 and 5) human HL-60 lysate with SIRT1 antibody. Predicted molecular weight ~80 kDa but is routinely observed at 110~120 kDa due to post-translational modification.
Flow cytometry testing of human HeLa cells with SIRT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SIRT1 antibody.

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NSJ-RQ5549-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SIRT1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids Y142-H533) was used as the immunogen for the SIRT1 antibody.
Limitation:
This SIRT1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
PM_Meta_Key:
Product_Images
PM_Meta_Value:
https://www.nsjbio.com/get_image.php?catalog_no=RQ5549&type=image1~Immunofluorescent staining of FFPE human HeLa cells with SIRT1 antibody (red) and Beta Tubulin mAb (green). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.|https://www.nsjbio.com/get_image.php?catalog_no=RQ5549&type=image2~Western blot testing of human 1) SiHa, 2) A549 and 3) Caco-2 cell lysate with SIRT1 antibody. Predicted molecular weight ~80 kDa but is routinely observed at 110~120 kDa due to post-translational modification.|https://www.nsjbio.com/get_image.php?catalog_no=RQ5549&type=image3~Western blot testing of human 1) HeLa, 2) 293T, 3) K562 and 4) MCF7 cell lysate with SIRT1 antibody. Predicted molecular weight ~80 kDa but is routinely observed at 110~120 kDa due to post-translational modification.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96EB6