NSJ Bioreagents

Filamin A Antibody / FLNA

Product Code:
 
NSJ-RQ5577
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Filamin A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 10
IHC staining of FFPE human skeletal muscle with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 10
IHC staining of FFPE human lung cancer with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 10
IHC staining of FFPE human lung cancer with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE mouse intestine with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE rat intestine with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE rat intestine with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 10
Immunofluorescent staining of FFPE human A549 cells with Filamin A antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 10
Western blot testing of human 1) A549, 2) U-2 OS, 3) HeLa and 4) PC-3 lysate with Filamin A antibody. Predicted molecular weight ~281 kDa.
9 / 10
Western blot testing of 1) rat ovary, 2) mouse lung and 3) mouse HEPA1-6 lysate with Filamin A antibody. Predicted molecular weight ~281 kDa.
10 / 10
Flow cytometry testing of human Caco-2 cells with Filamin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Filamin A antibody.
❮❯

IHC staining of FFPE human skeletal muscle with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with Filamin A antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with Filamin A antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) U-2 OS, 3) HeLa and 4) PC-3 lysate with Filamin A antibody. Predicted molecular weight ~281 kDa.
Western blot testing of 1) rat ovary, 2) mouse lung and 3) mouse HEPA1-6 lysate with Filamin A antibody. Predicted molecular weight ~281 kDa.
Flow cytometry testing of human Caco-2 cells with Filamin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Filamin A antibody.

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CodeSizePrice
NSJ-RQ5577-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Filamin A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Filamin A, alpha (FLNA) is a protein that in humans is encoded by the FLNA gene. It is mapped to Xq28. The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids E451-Q638) was used as the immunogen for the Filamin A antibody.
Limitation:
This Filamin A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P21333