NSJ Bioreagents

LPP Antibody / Lipoma preferred partner

Product Code:
 
NSJ-RQ5589
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the LPP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 6
IHC staining of FFPE human breast cancer with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human lung cancer with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse heart with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat heart with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 6
Flow cytometry testing of human A549 cells with LPP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LPP antibody.
6 / 6
Western blot testing of 1) human placenta, 2) rat ovary, 3) rat heart, 4) mouse ovary and 5) mouse heart lysate with LPP antibody. Predicted molecular weight: ~66 kDa but is routinely observed at 80~85 kDa.
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IHC staining of FFPE human breast cancer with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart with LPP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human A549 cells with LPP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LPP antibody.
Western blot testing of 1) human placenta, 2) rat ovary, 3) rat heart, 4) mouse ovary and 5) mouse heart lysate with LPP antibody. Predicted molecular weight: ~66 kDa but is routinely observed at 80~85 kDa.

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CodeSizePrice
NSJ-RQ5589-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LPP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
LPP (Lim Domain-Containing Preferred Translocation Partner in Lipoma), is a protein that in humans is encoded by the LPP gene. Petit et al. (1996) commented that the LPP-encoded protein should be classified as a novel member of the group 3 proteins of the LIM protein gene family. By partial cDNA cloning, Petit et al. (1996) established features of the genetic organization of LPP. The gene was found to span a genomic region of over 400 kb. By FISH and Southern blot analyses, Daheron et al. (2001) identified a rearrangement in the mixed lineage leukemia gene due to a novel t(3;11)(q28;q23) translocation in a patient who developed acute myeloid leukemia of the M5 type 3 years after treatment for a follicular lymphoma.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant protein (amino acids K10-C572) was used as the immunogen for the LPP antibody.
Limitation:
This LPP antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q93052