Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
POLRMT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
DNA-directed RNA polymerase, mitochondrial, MtRPOL, POLRMT
Isoforms
WB: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
POLRMT Antibody: This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. POLRMT mutations impair mitochondrial transcription causing neurological disease. Knock-down of MRPL12 by RNA interference results in instability of POLRMT.
- Inatomi et al. Biochim Biophys Acta Mol Cell Res. 2022; 1869(1); 119167
- Olahova et al. Nat Commun. 2021; 12(1):1135
- Nouws et al. J Biol Chem 2016; 291(2):989-997
POLRMT Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Predicted species reactivity based on immunogen sequence: Cow (82%); Chimpanzee (100%) ; monkey (82%); Cat (90%)
Anti-POLRMT antibody (9561) was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human POLRMT.
The immunogen is located within the last 50 amino acids of POLRMT.
Human POLRMT has 1 isoform (1230aa, 138.6kD). Mouse POLRMT has one isoform (1207aa, 136.7kD). Rat POLRMT has one isoform (1203aa, 135.8kD). 9561 can detect POLRMT in human, mouse and rat.
5442
RNA polymerase mitochondrial
POLRMT
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 139kD
Observed: 139 kD
O0411.2
50403691
POLRMT Antibody is affinity chromatography purified via peptide column.
Signal Transduction
O0411
Optimal dilutions for each application to be determined by the researcher.