ProSci

CD18 Antibody [1B4], Rabbit IgG

Product Code:
 
PSI-24-652
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1B4
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rhesus Macaque
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Radioimmunoassay (RIA)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Flow-cytometry using the anti-CD18 antibody 1B4 ( </b> Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of 1B4 (24-652- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Flow-cytometry using the anti-CD18 antibody 1B4 ( </b> Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of 1B4 (24-652- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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PSI-24-652-0.2mg0.2mg£681.00
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Further Information

Additional Names:
Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta, Complement receptor C3 subunit beta, ITGB2, MFI7, IB4, m1B4, mIB4
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest.
NCBI Gene ID #:
3689
NCBI Official Name:
integrin subunit beta 2
NCBI Official Symbol:
ITGB2
NCBI Organism:
Homo sapiens
Research Area:
Other
Swissprot #:
P05107
User NOte:
For research use only.